"Targeted Capture and Massively Parallel Sequencing for Genetic Testing of Breast and
Tomas Walsh, Research Asst. Professor with UW Medicine, describes the development of a comprehensive genetic test for inherited breast and ovarian cancer. The assay, called BROCA, uses DNA capture and next generation sequencing technology and is highly sensitive for identifying mutations in a panel of 21 tumor suppressor genes, including BRCA1, BRCA2, and other genes known to cause inherited breast or ovarian carcinoma. In the present study, BROCA is applied to analyze the germline DNA from 360 women undergoing surgery for primary ovarian, peritoneal, or fallopian tube carcinoma at the University of Washington.
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